Loss of centromere function drives karyotype evolution in closely related Malassezia species

eLife

Joseph Heitman¹  Giuseppe Ianiri²  Marco A Coelho²  Sheng Sun²  Kaustuv Sanyal²  Sundar Ram Sankaranarayanan³  Bhagya C Thimmappa³  Promit Ganguly³  Md Hashim Reza³  Christian Tellgren-Roth⁴  Thomas L Dawson Jnr⁵  Rakesh Netha Vadnala⁶  Rahul Siddharthan⁶ 

[1] Department of Drug Discovery, Medical University of South Carolina, School of Pharmacy, Charleston, United States;Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, United States;Molecular Mycology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru, India;National Genomics Infrastructure, Science for Life Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden;Skin Research Institute Singapore, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore;The Institute of Mathematical Sciences/HBNI, Chennai, India;

关键词: skin microbe;    chromosome fusion;    double-strand breaks;    kinetochore;    dicentric;   

DOI  :  10.7554/eLife.53944

来源: DOAJ

【 摘 要 】

Genomic rearrangements associated with speciation often result in variation in chromosome number among closely related species. Malassezia species show variable karyotypes ranging between six and nine chromosomes. Here, we experimentally identified all eight centromeres in M. sympodialis as 3–5-kb long kinetochore-bound regions that span an AT-rich core and are depleted of the canonical histone H3. Centromeres of similar sequence features were identified as CENP-A-rich regions in Malassezia furfur, which has seven chromosomes, and histone H3 depleted regions in Malassezia slooffiae and Malassezia globosa with nine chromosomes each. Analysis of synteny conservation across centromeres with newly generated chromosome-level genome assemblies suggests two distinct mechanisms of chromosome number reduction from an inferred nine-chromosome ancestral state: (a) chromosome breakage followed by loss of centromere DNA and (b) centromere inactivation accompanied by changes in DNA sequence following chromosome–chromosome fusion. We propose that AT-rich centromeres drive karyotype diversity in the Malassezia species complex through breakage and inactivation.

【 授权许可】

Unknown