An Ultra-Rapid Biosensory Point-of-Care (POC) Assay for Prostate-Specific Antigen (PSA) Detection in Human Serum

Sensors

Konstantina Kalogeropoulou¹  Georgios Giannakos¹  Athanasios Zafeirakis¹  Georgia Moschopoulou²  Athanasios Skevis²  Spyridon Kintzios²  Sophie Mavrikou² 

[1] Army Share Fund Hospital of Athens, Monis Petraki 10, 11521 Athens, Greece;Laboratory of Cell Technology, Faculty of Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855Athens, Greece;

关键词: bioelectric recognition assay;    cellular biosensor;    high-throughput;    immunoassay;    molecular identification through membrane engineering;    point-of-care;    prostate cancer;    prostate-specific antigen;   

DOI  :  10.3390/s18113834

来源: DOAJ

【 摘 要 】

Prostate-specific antigen (PSA) is the established routine screening tool for the detection of early-stage prostate cancer. Given the laboratory-centric nature of the process, the development of a portable, ultra rapid high-throughput system for PSA screening is highly desirable. In this study, an advancedpoint-of-care system for PSA detection in human serum was developed based on a cellular biosensor where the cell membrane was modified by electroinserting a specific antibody against PSA. Thirty nine human serum samples were used for validation of this biosensory system for PSA detection. Samples were analyzed in parallel with a standard immunoradiometric assay (IRMA) and an established electrochemical immunoassay was used for comparison purposes. They were classified in three different PSA concentration ranges (0, <4 and ≥4 ng/mL). Cells membrane-engineered with 0.25 μg/mL anti-PSA antibody demonstrated a statistically lower response against the upper (≥4 ng/mL) PSA concentration range. In addition, the cell-based biosensor performed better than the immunosensor in terms of sensitivity and resolution against positive samples containing <4 ng/mL PSA. In spite of its preliminary, proof-of-concept stage of development, the cell-based biosensor could be used as aninitiative for the development of a fast, low-cost, and high-throughput POC screening system for PSA.

【 授权许可】

Unknown