A Short-Term Response of Soil Microbial Communities to Cadmium and Organic Substrate Amendment in Long-Term Contaminated Soil by Toxic Elements

Frontiers in Microbiology

Michal Grunt¹  Daria Rapoport¹  Pavla Madrova¹  Yvona Smutna¹  Jan Kopecky¹  Marketa Sagova-Mareckova²  Marek Omelka³  Marek Vach⁴  Tomas Vetrovsky⁵  Petr Baldrian⁵ 

[1] Department of Epidemiology and Ecology of Microorganisms, Crop Research Institute, Prague, Czechia;Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czechia;Department of Probability and Mathematical Statistics, Faculty of Mathematics and Physics, Charles University, Prague, Czechia;Department of Water Resources and Environmental Modeling, Faculty of Environmental Sciences, Czech University of Life Sciences, Prague, Czechia;Laboratory of Environmental Microbiology, Institute of Microbiology of the AS CR, v.v.i., Prague, Czechia;

关键词: respiration;    diversity;    actinobacteria;    straw;    cellobiose;   

DOI  :  10.3389/fmicb.2018.02807

来源: DOAJ

【 摘 要 】

Two long-term contaminated soils differing in contents of Pb, Zn, As, Cd were compared in a microcosm experiment for changes in microbial community structure and respiration after various treatments. We observed that the extent of long-term contamination (over 200 years) by toxic elements did not change the total numbers and diversity of bacteria but influenced their community composition. Namely, numbers of Actinobacteria determined by phylum specific qPCR increased and also the proportion of Actinobacteria and Chloroflexi increased in Illumina sequence libraries in the more contaminated soil. In the experiment, secondary disturbance by supplemented cadmium (doses from double to 100-fold the concentration in the original soil) and organic substrates (cellobiose or straw) increased bacterial diversity in the less contaminated soil and decreased it in the more contaminated soil. Respiration in the experiment was higher in the more contaminated soil in all treatments and correlated with bacterial numbers. Considering the most significant changes in bacterial community, it seemed that particularly Actinobacteria withstand contamination by toxic elements. The results proved higher resistance to secondary disturbance in terms of both, respiration and bacterial community structure in the less contaminated soil.

【 授权许可】

Unknown