Distinct Serum and Fecal Metabolite Profiles Linking With Gut Microbiome in Older Adults With Frailty

Frontiers in Medicine

Fengliang Wang¹  Haoyu Zhang²  Yan Guo³  Jing Yu⁴  Yifan Lv⁴  Yi Jin⁴  Guoxian Ding⁴  Fan Xia⁴  Xin Chen⁴  Yan Mao⁴  Guoqin Zhu⁵ 

[1] Department of Breast Surgery, Nanjing Maternity and Child Health Care Hospital, The Affiliated Obstetrics and Gynaecology Hospital of Nanjing Medical University, Nanjing, China;Department of Human Biology Undergraduate, University of Toronto, Toronto, ON, Canada;Department of Neurology, Yancheng City No. 1 People’s Hospital, Yancheng, China;Division of Geriatric Endocrinology, Department of Geriatrics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China;Division of Geriatric Gastroenterology, Department of Geriatrics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China;

关键词: frailty;    healthy aging;    gut microbiota;    metabolites;    metabolomics;   

DOI  :  10.3389/fmed.2022.827174

来源: DOAJ

【 摘 要 】

Frailty is a critical aging-related syndrome but the underlying metabolic mechanism remains poorly understood. The aim of this study was to identify novel biomarkers and reveal potential mechanisms of frailty based on the integrated analysis of metabolome and gut microbiome. In this study, twenty subjects consisted of five middle-aged adults and fifteen older adults, of which fifteen older subjects were divided into three groups: non-frail, pre-frail, and frail, with five subjects in each group. The presence of frailty, pre-frailty, or non-frailty was established according to the physical frailty phenotype (PFP). We applied non-targeted metabolomics to serum and feces samples and used 16S rDNA gene sequencing to detect the fecal microbiome. The associations between metabolites and gut microbiota were analyzed by the Spearman’s correlation analysis. Serum metabolic shifts in frailty mainly included fatty acids and derivatives, carbohydrates, and monosaccharides. Most of the metabolites belonging to these classes increased in the serum of frail older adults. Propylparaben was found to gradually decrease in non-frail, pre-frail, and frail older adults. Distinct changes in fecal metabolite profiles and gut microbiota were also found among middle-aged adults, non-frail and frail older subjects. The relative abundance of Faecalibacteriu, Roseburia, and Fusicatenibacter decreased while the abundance of Parabacteroides and Bacteroides increased in frailty. The above altered microbes were associated with the changed serum metabolites in frailty, which included dodecanedioic acid, D-ribose, D-(-)-mannitol, creatine and indole, and their related fecal metabolites. The changed microbiome and related metabolites may be used as the biomarkers of frailty and is worthy of further mechanistic studies.

【 授权许可】

Unknown