Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.)

Journal of Integrative Agriculture

Cong-fen HE¹  Seong Sub Ku²  Xing-guo YE³  Shun-li WANG⁴  Pil Son Choi⁵  Suk Yoon Kwon⁶ 

[1] Correspondence YE Xing-guo, Tel: [phone], Fax: [phone];Department of Oriental Pharmaceutical Development, Nambu University, Gwangju 506-824, Korea;Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, P.R. China;Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China;Plant Systems Engineering Research Center, KRIBB, Daejeon 305-806, Korea;School of Sciences, Beijing Technology and Business University, Beijing 100048, P.R. China;

关键词: Cucumis sativus L.;    plant regeneration;    genetic transformation;    positive selection system;   

DOI  :  

来源: DOAJ

【 摘 要 】

Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major approach for cucumber, and its frequency has been up to 23%. For example, significantly enhancement of the transformation efficiency of this plant species was achieved from the cotyledon explants of the cultivar Poinsett 76 infected by Agrobacterium strains EHA105 with efficient positive selection system in lots of experiments. This review is to summarize some key factors influencing cucumber regeneration and genetic transformation, including target genes, selection systems and the ways of transgene introduction, and then to put forward some strategies for the increasing of cucumber transformation efficiency. In the future, it is high possible for cucumber to be potential bioreactor to produce vaccine and biomaterials for human beings.

【 授权许可】

Unknown