The significance of peripheral blood free CD147 and its induced product matrix metalloproteinase⁃9 in prognosis assessment of patients with traumatic brain injury

Chinese Journal of Contemporary Neurology and Neurosurgery

WEI Ming¹  LI Hong²  WANG De⁃sheng²  LIU Shi⁃min² 

[1] Department of Comprehensive Neurovascular Ward, Tianjin Huanhu Hospital, Tianjin 300350, China;Department of Neurosurgery, the Second Hospital of Tianjin Medical University, Tianjin 300211, China;

关键词: brain injuries;    traumatic;    extracellular matrix proteins;    matrix metalloproteinase 9;    prognosis;    enzyme⁃linked immunosorbent assay;   

DOI  :  10.3969/j.issn.1672⁃6731.2022.03.012

来源: DOAJ

【 摘 要 】

Objective To observe the dynamic changes of free CD147 and its induced product matrix metalloproteinase-9 (MMP-9) of patients with traumatic brain injury (TBI) in peripheral blood, and to explore the relationship between its expression changes and short-term prognosis. Methods Thirty-nine patients with TBI admitted to the Second Hospital of Tianjin Medical University from May 2014 to December 2016 were included in the study. The expression changes of free CD147 and MMP-9 in peripheral blood were determined by enzyme-linked immunosorbent assay (ELISA), and the activity of MMP-9 was determined through gelatin zymography. The prognosis was evaluated via Glasgow Outcome Scale (GOS) at discharge. Results There was no significant difference in GOS score between male and female patients at discharge (2.63±1.28 vs. 2.57±1.14; t = 0.161, P = 0.873). There was a statistically significant difference in GOS score between different age groups at discharge (t = 2.191, P = 0.038). The GOS scores of patients over 60 years old were lower than those of patients under 40 years old (t = 2.645, P = 0.014) and those between 40 and 60 years old (t = 2.320, P = 0.029). According to the GOS score, they were divided into a good prognosis group (GOS score ≥3, n = 25) and a poor prognosis group (GOS score < 3, n = 14). Free CD147 in peripheral blood of patients with poor prognosis [(5.07±1.89) ng/ml vs. (10.37±1.69）ng/ml; t = 2.080, P = 0.048] and MMP-9 [(41.55±4.67) ng/ml vs. (75.23±5.18) ng/ml; t = 2.512, P = 0.019] were higher than the good prognosis group. Correlation analysis showed that the GOS score at discharge was negatively correlated with free CD147 (r =﹣0.473, P = 0.000) and MMP-9 (r =﹣0.435, P = 0.036) in peripheral blood. Conclusions The higher the level of free CD147 and its induced product MMP-9 in peripheral blood of patients with TBI, the worse the prognosis, which indicated that free CD147 and MMP-9 can be used as important indicators for poor prognosis of patients with TBI.

【 授权许可】

Unknown