Clinical Grade Human Adipose Tissue-Derived Mesenchymal Stem Cell Banking

Acta Medica Iranica

Abbas Norouzi-Javidan¹  Fereshteh Mohamadi-Jahani²  Kazem Bidaki²  Forough Azam Sayahpour³  Ahmad Reza Dehpour⁴  Bagher Larijani⁵  Babak Arjmand⁶  Parisa Goodarzi⁷  Khadijeh Fallahzadeh⁸  Hamidreza Aghayan⁸ 

[1] Brain and Spinal Cord Injury Research Center, Cellular Fanavaran Knowledge-Based Organization, Tehran University of Medical Sciences, Tehran, Iran.;Department of Biology, Payame Noor University, Tehran, Iran.;Department of Molecular Systems Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.;Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.;Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran Iran.;Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.;School of Nursing and Midwifery, Iran University of Medical Sciences, Tehran, Iran.;cGMP Grade Stem Cell Facility, Chronic Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.;

关键词: Adipose-derived stem cell;    Cell therapy;    Good manufacturing practice;    Mesenchymal stem cells;   

DOI  :  

来源: DOAJ

【 摘 要 】

In this study, our aim was to produce a generation of GMP-grade adipose tissue-derived mesenchymal stem cells for clinical applications. According to our results, we fulfill to establish consistent and also reproducible current good manufacturing practice (cGMP) compliant adipose tissue-derived mesenchymal stem cells from five female donors. The isolated cells were cultured in DMEM supplemented with 10% fetal bovine serum and characterized by standard methods. Moreover, karyotyping was performed to evaluate chromosomal stability. Mean of donors’ age was 47.6 ± 8.29 year, mean of cell viability was 95.6 ± 1.51%, and cell count was between 9×106 and 14×106 per microliter with the mean of 12.2×106 ± 2863564.21 per microliter. The main aim of this project was demonstrating the feasibility of cGMP-compliant and clinical grade adipose tissue-derived mesenchymal stem cells preparation and banking for clinical cell transplantation trials.

【 授权许可】

Unknown