Biotechnology & Biotechnological Equipment | |
Assessment of the genetic diversity and population structure of Sophora tonkinensis in South China by AFLP markers | |
Kun-Hua Wei1  Zhu Qiao2  Chanthaphoone Keovongkod3  Dong Xiao3  Long-Fei He3  | |
[1] Department of Conservation Center of Medicinal Plants, Guangxi Key Laboratory of Medicinal Resources Conservation and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plants;Key Laboratory of Plant Development and Environmental Adaption Biology, Ministry of Education, School of Life Science, Shandong University;National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University; | |
关键词: aflp; genetic diversity; population structure; sophora tonkinensis; | |
DOI : 10.1080/13102818.2020.1812430 | |
来源: DOAJ |
【 摘 要 】
Sophora tonkinensis is an important source of herbal medicine for treatment of swelling and pain in the throat. Its chemical composition has been extensively studied; however, there are still gaps in the research on genetic diversity of S. tonkinensis which can provide insights for better utilization and conservation of genetic resources. In this study, 23 populations of diverse locations from southern China were evaluated using AFLP markers. The average number of alleles (Na and Ne) from all populations was 0.608 (Na) and 1.153 (Ne), average Shannon’s information index was 0.137, which indicated that the genetic diversity of the whole populations was low. Unweighted pair-group method with arithmetic mean (UPGMA) clustering and principal coordinates analysis (PCoA) showed that the populations could be divided into three clades. The analysis of molecular variance (AMOVA) indicated that 20% of the molecular variance was due to differences among wild populations and 80% of the molecular variance was due to divergence within wild populations. There was a significant positive correlation between genetic distance and geographical distance, as well as ecological factors including altitude and temperature. It is necessary to closely monitor the status of the wild populations and consider expanding their genetic diversity. Establishment of the conservation basis in different areas is more conducive to the protection of S. tonkinensis.
【 授权许可】
Unknown