Use of Unamplified RNA/cDNA–Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses

Emerging Infectious Diseases

关键词: Ebola virus;    nanopore sequencing;    Venezuelan equine encephalitis virus;    RNA virus;    genomics;    sequencing;   

DOI  :  10.3201/eid2208.160270

来源: DOAJ

【 摘 要 】

Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezuelan equine encephalitis virus and Ebola virus in 3 hours from sample receipt to data acquisition, demonstrating a fieldable technique for RNA virus characterization.

【 授权许可】

Unknown