International Journal of Molecular Sciences | |
Trp Fluorescence Redshift during HDL Apolipoprotein Denaturation Is Increased in Patients with Coronary Syndrome in Acute Phase: A New Assay to Evaluate HDL Stability | |
Rosalinda Posadas-Sánchez1  Gilberto Vargas-Alarcón2  María Luna-Luna2  José Manuel Fragoso2  Victoria López-Olmos2  Rocío Bautista-Pérez2  Elizabeth Carreón-Torres2  Óscar Pérez-Méndez2  Héctor González-Pacheco3  | |
[1] Department of Endocrinology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City 14080, Mexico;Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City 14080, Mexico;Emergency Department, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City 14080, Mexico; | |
关键词: Trp fluorescence; HDL stability; atherosclerosis; protein structure; coronary heart disease; | |
DOI : 10.3390/ijms22157819 | |
来源: DOAJ |
【 摘 要 】
High-density lipoproteins’ (HDL) stability is a determinant of their residence times in plasma and consequently an important parameter that influences the beneficial properties of these lipoproteins. Since there are no accessible procedures for this purpose, here, we describe the methodological conditions to assess the stability of the HDL based on the redshift of the fluorescence spectrum of tryptophans contained in the structure of HDL-apolipoproteins during incubation with urea 8M. Along the HDL denaturation kinetics, the main variations of fluorescence were observed at the wavelengths of 330, 344, and 365 nm at room temperature. Therefore, HDL denaturation was estimated using the tryptophan (Trp)-ratio of fluorescence intensity (
【 授权许可】
Unknown