Oligostilbenes extracts from Iris lactea Pall. var. chinensis (Fisch.) Koidz improve lipid metabolism in HFD/STZ-induced diabetic mice and inhibit adipogenesis in 3T3-L1 cells

Biomedicine & Pharmacotherapy

Gang Li¹  Zhenhua Wang²  Na Hu²  Fangfang Tie³  Honglun Wang³  Ji Li³ 

[1] University of Chinese Academy of Science, Beijing, 100049, PR China;Center for Mitochondrial and Healthy Aging, College of Life Science, Yantai University, Yantai, 264005, PR China;Key Laboratory of Tibetan Medicine Research, Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, 810008, PR China;

关键词: Oligostilbenes extracts;    Type 2 diabetes;    Lipid metabolism;    3T3-L1 cells;    Anti-adipogenesis;   

DOI  :  

来源: DOAJ

【 摘 要 】

The present study investigated the anti-diabetic effects of Oligostilbenes extracts (Olie) from Iris lactea Pall. var. chinensis (Fisch.) Koidz (I. lactea) and the potential mechanisms, in high-fat-diet (HFD)-induced diabetic mice and 3T3-L1 adipocytes. Olie are a group of major active extracts from I. lactea that have been used as nutraceutical because of their antioxidant activity. Six-week Olie treatment improved fasting blood glucose levels, as well as blood lipid profiles in HFD/streptozocin (STZ)-induced diabetic mice, compared with non-treated mice. Olie treatment upregulated the levels of phosphorylated of AMPK and lipolysis-related proteins, while the hepatic expression of ACC and FAS in diabetic mice was inhibited. In cultured 3T3-L1 cells, Olie (2−15 μg/mL) treatment dose-dependently suppressed the differentiation into mature adipocytes and lowered cellular lipid accumulation. Consistently, Olie reduced expression of adipogenic transcription factors including CCAAT/enhancer-binding protein β (C/EBPβ) and peroxisome proliferator-activated receptor γ (PPARγ). In addition, mitochondrial function in 3T3-L1 adipocytes was improved after Olie treatment. Taken together, our findings indicate that a lipid-lowering effect of Olie in HFD/STZ-induced diabetic mice and adipogenesis/ lipogenesis suppressing effect in 3T3-L1 cells, via regulating the expression of lipid metabolism-related proteins.

【 授权许可】

Unknown