Correlation Between Pathogenic Determinants Associated with Clinically Isolated Non-Typhoidal Salmonella

Pathogens

Ching-Chuan Liu¹  Tzong-Shiann Ho¹  Yu-Chung Chiang²  Tsyr-Huei Chiou³  Hao-Ven Wang³  BoimpoundiEunice Flavie Ouali³  I-Chu Lin³  Jenn-Wei Chen⁴ 

[1] Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan 701, Taiwan;Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan;Department of Life Sciences, National Cheng Kung University, Tainan City 701, Taiwan;Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan;

关键词: non-typhoidal Salmonella;    virulence factor;    pathogenicity;    clinical isolates;   

DOI  :  10.3390/pathogens10010074

来源: DOAJ

【 摘 要 】

Non-typhoidal and Typhoidal Salmonella are bacterial pathogens source of worldwide and major disease burden. Virulent determinants of specific serovars belonging to non-typhoidal Salmonella have been extensively studied in different models, yet the pathogenesis of this group of bacteria and the development of clinical symptoms globally remains underexplored. Herein, we implemented microbiological and molecular procedures to investigate isolate virulence traits and molecular diversity, likely in association with disease severity. Our results show that selected clinical isolates from a tertiary referring hospital, depending on the richness of the environment and isolate serotypes, exhibited different, and sometimes controversial, virulence properties. The tested strains were susceptible to Ceftriaxone (90%) with decreasing reactivity to Trimethoprim–Sulfamethoxazole (72%), Chloramphenicol (64%), Ampicillin (48%), Gentamicin (44%), and Ciprofloxacin (2%). Disc susceptibility results partially correlated with minimum inhibitory concentration (MIC); however, special attention must be given to antimicrobial treatment, as a rise in multi-resistant isolates to Trimethoprim–Sulfamethoxazole (2/38 µg/mL), Minocycline (8 µg/mL) and Ampicillin (16 µg/mL) has been noticed, with two isolates resistant to Ceftazidime (16 µg/mL). By comparison to previous molecular epidemiology studies, the variation in the gene profiles of endemic pathogens supports the need for continuous and up-to-date microbiological and molecular reports.

【 授权许可】

Unknown