A putative N-BAR-domain protein is crucially required for the development of hyphae tip appressorium-like structure and its plant infection in Magnaporthe oryzae

Phytopathology Research

Lili Lin¹  Si Chen¹  Xuewen Chen¹  Ammarah Shabbir¹  Zonghua Wang¹  Justice Norvienyeku¹  Xiaomin Chen¹ 

[1] State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, College of Life Science, Fujian Agriculture and Forestry University;

关键词: Magnaporthe oryzae;    Peripheral membrane protein;    Appressorium-like structure;    N-BAR domain;    Membrane tubulation;   

DOI  :  10.1186/s42483-019-0038-2

来源: DOAJ

【 摘 要 】

Abstract Membrane remodeling modulates many biological processes. The binding of peripheral proteins to lipid membranes results in membrane invaginations and protrusions, which regulate essential intra-cellular membrane and extra-cellular trafficking events. Proteins that bind and re-shape bio-membranes have been identified and extensively investigated. The Bin/Amphiphysin/Rvs (BAR) domain proteins are crescent-shape and play a conserved role in tubulation and sculpturing of cell membranes. We deployed targeted gene replacement technique to functionally characterize two hypothetical proteins (MoBar-A and MoBar-B) containing unitary N-BAR domain in Magnaporthe oryzae. The results obtained from phenotypic examinations showed that MoBAR-A deletion exerted a significant reduction in the growth of the defective ∆Mobar-A strain. Also, MoBAR-A disruption exclusively compromised hyphae-mediated infection. Additionally, the targeted replacement of MoBAR-A suppressed the expression of genes associated with the formation of hyphae tip appressorium-like structure in M. oryzae. Furthermore, single as well as combined deletion of MoBAR-A and MoBAR-B down-regulated the expression of nine different membrane-associated genes. From these results, we inferred that MoBAR-A plays a key and unique role in the pathogenesis of M. oryzae through direct or indirect regulation of the development of appressorium-like structures developed by hyphae tip. Taken together, these results provide unique insights into the direct contribution of the N-BAR domain proteins to morphological, reproduction, and infectious development of M. oryzae.

【 授权许可】

Unknown