The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris

Food Science and Human Wellness

Tai-Sheng Yeh¹  Chun-Ren Hsieh²  Fang-Rong Chang³  Yi-Hong Tsai³  Yang-Chang Wu⁴  Ying-Chen Lu⁵  Chien-Kei Wei⁵  Chi-Ying Li⁵  Wei-Kuang Lai⁵ 

[1] Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan, China;Department of Food Science and Nutrition, Meiho University, Pingtung 912, Taiwan, China;Department of Food Science, National Chiayi University, Chiayi City 600, Taiwan, China;Graduate Institute of Integrated Medicine, China Medical University, Taichung 404, Taiwan, China;Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan, China;

关键词: Medicinal fungi;    Protoplast fusion;    Antrodia cinnamomea;    Cordyceps militaris;    Secondary metabolite;   

DOI  :  

来源: DOAJ

【 摘 要 】

This study generated two fused protoplasts of Antrodia cinnamomea and Cordyceps militaris in two ways. The protoplasts of A. cinnamomea were inactivated by heat to inactivate biochemical processes and enzymatic activities in the cytoplasm, and the protoplasts of C. militaris were inactivated by UV radiation to invalidate their genome function, then they were fused under optimal conditions to get a fusion rate as (7.42 ± 0.8) × 10-6 fusants/mL; the new fusants were abbreviated as Ac-Cm. On the other hand, when A. cinnamomea and C. militaris were treated with heat and UV oppositely using similar experiments, the fusion rate was (9.70 ± 0.68) × 10-5 fusants/mL, and the new fusants were abbreviated as Cm-Ac. We selected each of two best-growing fused colonies Ac-Cm-1, Ac-Cm-2, Cm-Ac-1, and Cm-Ac-2, together with parental A. cinnamomea and C. militaris, and studied their morphology, growth antagonism tests, and genetic relationships by 18S rRNA sequencing. In comparison with the initial cultures of 4 fusants, the yields of adenosine, biomass, cordycepic acid, cordycepin, total polysaccharide, and total triterpenoids were increased up 1.305−50.1563 times in the optimal medium conditions. For gene stability tests, those of the four fusants and their outputs were stabilized within 10 generations.

【 授权许可】

Unknown