Effect of low level laser and low intensity pulsed ultrasound therapy on bone remodeling during orthodontic tooth movement in rats

Progress in Orthodontics

Ahmad Azlina¹  Rumaizi Shaari²  M. S. Salzihan³  Mohammad Khursheed Alam⁴  Rozita Hassan⁵  Mohammed Mahmood Jawad Alazzawi⁶  Adam Husein⁷ 

[1]Biochemistry/Molecular biology, School of Dental Sciences, Universiti Sains Malaysia

[2]Clinical sciences, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan

[3]Department of Pathology, School of Medical sciences, Universiti Sains Malaysia

[4]Orthodontic Department, College of Dentistry, Al Jouf University

[5]Orthodontic Unit, School of Dental Sciences, Universiti Sains Malaysia

[6]Orthodontics and Dentofacial Orthopedics, Eastman Institute for Oral Health, University of Rochester Medical Center

[7]School of Dental Sciences, Universiti Sains Malaysia

关键词: Orthodontics;    Laser;    Ultrasound;    Bone remodeling;    Tooth movement;   

DOI  :  10.1186/s40510-018-0208-2

来源: DOAJ

【 摘 要 】

Abstract Background Quality bone regeneration, which leads to the improvement of bone remodeling, is essential for orthodontic treatment. In order to improve bone regeneration and increase the amount of tooth movement, different techniques have been implemented. The object of this study is to compare the effects of low-level laser therapy (LLLT), low-intensity pulsed ultrasound (LIPUS), and their combination on bone remodeling during orthodontic tooth movement. Methods Eighty (80) male, 6-week-old Sprague Dawley rats were grouped in to four groups, the first group was irradiated with (940 nm) diode laser, second group with LIPUS, and third group with combination of both LLLT and LIPUS. A forth group used was a control group in an incomplete block split-mouth design. The LLLT and LIPUS were used to treat the area around the moving tooth once a day on days 0–7, then the experiment was ended in each experimental endpoint (1, 3, 7, 14, and 21 days). For amount of tooth movement, models were imaged and analyzed. Histological examination was performed after staining with (hematoxylin and eosin) and (alizarin red and Alcian Blue) stain. One step reverse transcription-polymerase chain reaction RT-PCR was also performed to elucidate the gene expression of RANK, RANKL, OPG, and RUNX-2. Results The amount of tooth movement, the histological bone remodeling, and the RT-PCR were significantly greater in the treatment groups than that in the control group. Among the treatment groups, the combination group was the highest and the LIPUS group was the lowest. Conclusion These findings suggest that LLLT and LIPUS can enhance the velocity of tooth movement and improve the quality of bone remodeling during orthodontic tooth movement.

【 授权许可】

Unknown