Population Diversity of Antibiotic Resistant Enterobacterales in Samples From Wildlife Origin in Senegal: Identification of a Multidrug Resistance Transposon Carrying blaCTX–M–15 in Escherichia coli

Frontiers in Microbiology

R. Adriana Hernandez-Aguilar¹  Amanda Barciela²  Didier Raoult³  Edmond Kuete Yimagou³  Oleg Mediannikov³  Rim Abdallah³  Bernard Davoust³  Sophie Alexandra Baron³  Linda Hadjadj³  Ahmad Ibrahim³  Jean-Marc Rolain³  Cheikh Sokhna⁵  Georges Diatta⁵ 

[1] Department of Social Psychology and Quantitative Psychology, Faculty of Psychology, Serra Hunter Programme, University of Barcelona, Barcelona, Spain;Dindefelo Biological Station, Jane Goodall Institute Spain and Senegal, Kedougou, Senegal;IHU Méditerranée Infection, Marseille, France;IRD, APHM, MEPHI, Faculté de Médecine et de Pharmacie, Aix Marseille University, Marseille, France;VITROME IRD 257, Campus International de Recherche IRD-UCAD de Hann, Dakar, Senegal;

关键词: antibiotic resistance;    Enterobacterales;    primates;    environment;    Senegal;   

DOI  :  10.3389/fmicb.2022.838392

来源: DOAJ

【 摘 要 】

IntroductionThe role of wildlife in the transmission of antimicrobial resistant (AMR) is suspected but scarcely reported in current studies. Therefore, we studied the dynamics and prevalence of antibiotic-resistant Enterobacterales in antibiotic-limited areas of Senegal.Materials and MethodsWe collected fecal samples from monkeys and apes (N = 226) and non-fecal environmental samples (N = 113) from Senegal in 2015 and 2019. We grew the samples on selective media, subsequently isolated AMR Enterobacterales, and then sequenced their genomes.ResultsWe isolated 72 different Enterobacterales among which we obtained a resistance rate of 65% for colistin (N = 47/72) and 29% for third generation-cephalosporin (C3G) (29%, N = 21/72). Interestingly, almost 46% of our isolates, among Enterobacter sp., Citrobacter cronae and Klebsiella aerogenes, belong to 34 new STs. Moreover, the genes blaCTX–M–15, blaTEM1B, sul2, dfrA14, qnrs, aph(3′′), aph(6), tetA, and tetR harbored within a transposon on the IncY plasmid of ST224 Escherichia coli were transferred and inserted into a ST10 E. coli phage coding region.ConclusionWildlife constitutes a rich, unexplored reservoir of natural microbial diversity, AMR genes and international resistant clones pathogenic in humans. The presence of a transposon that carries AMR genes is intriguing since no antibiotics are used in the non-human primates we studied.

【 授权许可】

Unknown