Evaluation of antiradical assays used in determining the antioxidant capacity of pure compounds and plant extracts

Química Nova

Edmar Silva Sobral¹  Oriana Aparecida Fávero¹  Paulete Romoff¹  Marcelo José Pena Ferreira¹  Thuany Alves Silva²  Sandro de Oliveira²  Wilhelm Josef Baader²  Camila Rodrigues Eckert²  Glalci Alves de Souza² 

[1] Universidade Presbiteriana Mackenzie;Universidade de São Paulo;

关键词: luminol chemiluminescence;    antioxidant assays;    Trolox® percentage.;   

DOI  :  10.5935/0100-4042.20140076

来源: DOAJ

【 摘 要 】

The efficiency of the chemiluminescence luminol method and colorimetric DPPH and ABTS methods in evaluating the antiradical capacity of pure compounds and plant extracts with antioxidant potential is compared. In case of pure compounds, the values of parameter 'n' (number of radicals quenched per molecule of antiradical) for ascorbic acid, p-hydroquinone, catechol, quercetin, and rutin are similar when measured by colorimetric assays; however, considerably lower values of n are obtained with the luminol assay. The antiradical activity of extracts from male and female individuals of Baccharis burchelli and Baccharis crispa were determined by the luminol assay and expressed using the new Trolox® percentage (%Trolox®) parameter.

【 授权许可】

Unknown