Heparin-binding protein in sputum as a marker of pulmonary inflammation, lung function, and bacterial load in children with cystic fibrosis

BMC Pulmonary Medicine

Fredrik Kahn¹  Gisela Hovold¹  Lisa I. Påhlman¹  Arne Egesten²  Victoria Palmcrantz³ 

[1] Department of Clinical Sciences Lund, Division of Infection Medicine, BMC B14, Lund University, Skåne University Hospital;Department of Clinical Sciences Lund, Respiratory Medicine & Allergology, Lund University;Skåne University Hospital, Clinic of Paediatrics;

关键词: Cystic fibrosis;    Sputum;    Inflammation;    Airway infection;    Lung function;    Children;   

DOI  :  10.1186/s12890-018-0668-7

来源: DOAJ

【 摘 要 】

Abstract Background Cystic fibrosis (CF) is associated with bacterial pulmonary infections and neutrophil-dominated inflammation in the airways. The aim of this study was to evaluate the neutrophil-derived protein Heparin-binding protein (HBP) as a potential sputum marker of airway inflammation and bacterial load. Methods Nineteen CF patients, aged 6–18 years, were prospectively followed for 6 months with sputum sampling at every visit to the CF clinic. A total of 41 sputum samples were collected. Sputum-HBP was analysed with ELISA, neutrophil elastase activity with a chromogenic assay, and total bacterial load with RT-PCR of the 16 s rDNA gene. Data were compared to lung function parameters and airway symptoms. Results HBP and elastase correlated to a decrease in FEV1%predicted compared to the patients´ individual baseline pulmonary function (∆FEV1), but not to bacterial load. Area under the receiver operating characteristic curve values for the detection of > 10% decrease in ∆FEV1 were 0.80 for HBP, 0.78 for elastase, and 0.54 for bacterial load. Conclusions Sputum HBP is a promising marker of airway inflammation and pulmonary function in children with CF.

【 授权许可】

Unknown