Extracellular microRNAs: key players to explore the outcomes of in vitro fertilization

Reproductive Biology and Endocrinology

Ahmed M. Isa¹  Hooria Younas²  Zahira Hassan³  Celal Kaloglu⁴  Ender Yalcinkaya Kalyan⁵  Hikmet Hakan Aydin⁶  Aysegul Yildiz⁷  Bilgün Oztürk Turhan⁸  Shahzad Bhatti⁹  Sana Abbas⁹  Haroon Latif Khan⁹  Yousaf Latif Khan⁹  Rachel Ziders^1,10 

[1] Assisted Conception Unit, Obstetrics and Gynecology Department, College of Medicine, King Saud University;Department of Biochemistry, Kinnaird College Lahore;Department of Cellular Pathology, Royal Free Hospital;Department of Histology and Embryology, Cumhuriyet University Faculty of Medicine;Department of IVF unit, Private Adatip Hospital;Department of Medical Biochemistry, Ege University School of Medicine;Department of Molecular Biology and Genetics, Faculty of Science, Mugla Sitki Kocman University;Department of Obstetrics and Gynecology, Nisdetiye Maha;Lahore Institute of Fertility and Endocrinology, Hameed Latif Hospital;Your Family Fertility;

关键词: PCOS;    miRNA expression;    Follicular fluid;    IVF;    Embryo quality;   

DOI  :  10.1186/s12958-021-00754-9

来源: DOAJ

【 摘 要 】

Abstract Background MicroRNAs (miRNAs) are small RNA molecules that modulate post-transcriptional gene regulation. They are often used as promising non-invasive biomarkers for the early diagnosis of cancer. However, their roles in assisted reproduction are still unknown. Methods This prospective study was designed to evaluate the expression profiles of seven extracellular miRNAs (miR-7-5p, miR-202-5p, miR-378-3p, miR-224, miR-320a, miR-212-3p, and miR-21-5p) in human follicular fluid (FF) to explore the outcomes of in vitro fertilization (IVF). Of 255 women, 145 were without polycystic ovary syndrome (PCOS), and their ovarian assets were normal (NOR), while 110 were with normo-androgenic PCOS. Results The combination of six FF miRNAs expression profile discriminated between PCOS and NOR women with a sensitivity of 79.2% and a specificity of 87.32% (AUC = 0.881 [0.61; 0.92], p = 0.001). MiR-202-5p significantly had a lower abundance level, and miR-378-3p had a high abundance level in pooled FF samples from patients treated with human menopausal gonadotropin (hMG) than those treated with recombinant follicle-stimulating hormone (rFSH) (p < 0.001). Our results showed that miRNA-320a was significantly different in top-quality embryos versus non-top-quality embryos on day 3 in NOR patients with a sensitivity of 80% and specificity of 71%, (AUC = [0.753 (0.651; 0.855)], p = 0.001). For clinical pregnancy outcome prediction, FF miRNA-21 exhibited high sensitivity (74.8%) and specificity (83.7%) with the AUC value of 0.774 (0.682; 0.865). Conclusion Conclusively, our results provide evidence that miR-7-5p, miR-378-3p, miR-224, miR-212-3p were a differentially high expression in normo-androgenic PCOS patients than NOR patients. While miRNA-320a was significantly different in top-quality embryos versus non-top-quality embryos on day 3 (p = 0.001). The expression level of FF miR-212-3p was significantly related to the probability of embryos to develop into a high-quality blastocyst in patients with normal ovarian reserve.

【 授权许可】

Unknown