Polyethylene Glycol6000/carbon Nanodots as Fluorescent Bioimaging Agents

Nanomaterials

Chien-Te Hsieh¹  Chun-Chieh Fu²  Ruey-Shin Juang²  Tai-Hao Hsu³  Shih-Fu Ou⁴  Shyi-Tien Chen⁵  Yi-Huang Hsueh⁶  Chien-Hui Wu⁶  Chun-Yung Wu⁷  Chih-Ching Chien⁷ 

[1] Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan 32003, Taiwan;Department of Chemical and Materials Engineering, Chang Gung University, Guishan, Taoyuan 33302, Taiwan;Department of Food Science and Biotechnology, Da-Yeh University, Changhua 51591, Taiwan;Department of Mold and Die Engineering, National Kaohsiung University of Science and Technology, Kaohsiung 80778, Taiwan;Department of Safety, Health and Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung 81164, Taiwan;Department of SeaFood Science, National Kaohsiung University of Science Kaohsiung 81157, Taiwan;Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Taoyuan 32003, Taiwan;

关键词: carbon quantum dots;    nanomaterials;    bioimaging;    photoluminescence;    hydrothermal synthesis;   

DOI  :  10.3390/nano10040677

来源: DOAJ

【 摘 要 】

Photoluminescent nanomaterials have immense potential for use in biological systems due to their excellent fluorescent properties and small size. Traditional semiconductor quantum dots are heavy-metal-based and can be highly toxic to living organisms, besides their poor photostability and low biocompatibility. Nano-sized carbon quantum dots and their surface-modified counterparts have shown improved characteristics for imaging purposes. We used 1,3, 6-trinitropyrene (TNP) and polyethylene glycol₆₀₀₀ (PEG₆₀₀₀) in a hydrothermal method to prepare functional polyethylene glycol₆₀₀₀/carbon nanodots (PEG₆₀₀₀/CDs) and analyzed their potential in fluorescent staining of different types of bacteria. Our results demonstrated that PEG₆₀₀₀/CDs stained the cell pole and septa of gram-positive bacteria B. Subtilis and B. thuringiensis but not those of gram‐negative bacteria. The optimal concentration of these composite nanodots was approximately 100 ppm and exposure times varied across different bacteria. The PEG₆₀₀₀/CD composite had better photostability and higher resistance to photobleaching than the commercially available FM4-64. They could emit two wavelengths (red and green) when exposed to two different wavelengths. Therefore, they may be applicable as bioimaging molecules. They can also be used for differentiating different types of bacteria owing to their ability to differentially stain gram-positive and gram-negative bacteria.

【 授权许可】

Unknown