Non-HLA Antibodies in Hand Transplant Recipients Are Connected to Multiple Acute Rejection Episodes and Endothelial Activation

Journal of Clinical Medicine

Kai Schulze-Forster¹  Harald Heidecke¹  Rusan Catar²  Dorota Kamińska³  Mirosław Banasik³  Mariusz Kusztal³  Andrzej Oko⁴  Katarzyna Korybalska⁵  Joanna Łuczak⁵  Rafał Rutkowski⁵  Włodzimierz Samborski⁶  Dorota Sikorska⁶  Przemysław Daroszewski⁶  Jerzy Jabłecki⁷ 

[1] CellTrend GmbH, 14943 Luckenwalde, Germany;Department of Nephrology and Internal Intensive Care Medicine, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10117 Berlin, Germany;Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, 50-556 Wroclaw, Poland;Department of Nephrology, Transplantology and Internal Medicine, Poznan University of Medical Sciences, 60-355 Poznan, Poland;Department of Pathophysiology, Poznan University of Medical Sciences, 60-806 Poznan, Poland;Department of Rheumatology, Rehabilitation and Internal Medicine, Poznan University of Medical Sciences, 61-545 Poznan, Poland;Subdepartment of Replantation of Limbs, St. Hedwig of Silesia Hospital, 55-100 Trzebnica, Poland;

关键词: hand transplantation;    non-HLA antibodies;    rejection;    vasculopathy;    vascularized composite allotransplantation;   

DOI  :  10.3390/jcm11030833

来源: DOAJ

【 摘 要 】

The role of anti-HLA antibodies in transplant rejection is well-known but the injury associated with non-HLA antibodies is now widely discussed. The aim of our study was to investigate a role of non-HLA antibodies in hand allografts rejection. The study was performed on six patients after hand transplantation. The control group consisted of: 12 kidney transplant recipients and 12 healthy volunteers. The following non-HLA antibodies were tested: antibody against angiotensin II type 1 receptor (AT1R-Ab), antibody against endothelin-1 type-A-receptor (ETAR-Ab), antibody against protease-activated receptor 1 (PAR-1-Ab) and anti-VEGF-A antibody (VEGF-A-Ab). Chosen proinflammatory cytokines (Il-1, IL-6, IFNγ) were used to evaluate the post-transplant humoral response. Laboratory markers of endothelial activation (VEGF, sICAM, vWF) were used to assess potential vasculopathy. The patient with the highest number of acute rejections had both positive non-HLA antibodies: AT1R-Ab and ETAR-Ab. The same patient had the highest VEGF-A-Ab and very high PAR1-Ab. All patients after hand transplantation had high levels of laboratory markers of endothelial activation. The existence of non-HLA antibodies together with multiple acute rejections observed in patient after hand transplantation should stimulate to look for potential role of non-HLA antibodies in humoral injury in vascular composite allotransplantation.

【 授权许可】

Unknown