In Vitro Cellular Uptake Studies of Self-Assembled Fluorinated Nanoparticles Labelled with Antibodies

Nanomaterials

Carolina Carrillo-Carrion¹  Wolfgang J. Parak¹  Mónica Carril¹  Khosro Khajeh²  Mona Atabakhshi-Kashi²  Hossein Mahdavi³ 

[1] Bioengineered Particles Group, CIC biomaGUNE, 20014 San Sebastian, Spain;Department of Nanobiotechnology, Tarbiat Modares University, Tehran 14115-175, Iran;School of Chemistry, College of Science, University of Tehran, Tehran 14174-66191, Iran;

关键词: nanoparticles;    quantum dots;    antibody conjugation;    fluorination;    self-assembly;   

DOI  :  10.3390/nano11081906

来源: DOAJ

【 摘 要 】

Nanoparticles (NPs) functionalized with antibodies (Abs) on their surface are used in a wide range of bioapplications. Whereas the attachment of antibodies to single NPs to trigger the internalization in cells via receptor-mediated endocytosis has been widely studied, the conjugation of antibodies to larger NP assemblies has been much less explored. Taking into account that NP assemblies may be advantageous for some specific applications, the possibility of incorporating targeting ligands is quite important. Herein, we performed the effective conjugation of antibodies onto a fluorescent NP assembly, which consisted of fluorinated Quantum Dots (QD) self-assembled through fluorine–fluorine hydrophobic interactions. Cellular uptake studies by confocal microscopy and flow cytometry revealed that the NP assembly underwent the same uptake procedure as individual NPs; that is, the antibodies retained their targeting ability once attached to the nanoassembly, and the NP assembly preserved its intrinsic properties (i.e., fluorescence in the case of QD nanoassembly).

【 授权许可】

Unknown