Potential prognostic biomarkers related to immunity in clear cell renal cell carcinoma using bioinformatic strategy

Bioengineered

Mingyao Li¹  Zhanchun Zhang¹  Zhenfei Xiang¹  Danfei Hu¹  Erdong Shen²  Senquan Yu³ 

[1] Ningbo Medical Center Lihuili Hospital, Ningbo University;The First People's Hospital of Yueyang;The Second Affiliated Hospital of Zhejiang Chinese Medical University;

关键词: clear cell renal cell carcinoma;    wgcna;    prognostic biomarker;    immune infiltration;   

DOI  :  10.1080/21655979.2021.1924546

来源: DOAJ

【 摘 要 】

The clear cell renal cell carcinoma (ccRCC) is the main pathological subtype of renal cell carcinoma. Immune system evasion, one hallmark of cancer, contributes to cancer cells in escaping from the attack of immune cells. In order to identify potential prognostic biomarkers in ccRCC patients and immune cells fraction, we collected and downloaded profiles from The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) database. We obtained 2 modules significantly associated with tumor stage and immune cells; functional enrichment analysis showed that genes in the module ‘yellow’ were significantly enriched in proteins targeting to membrane and ribosome, as well as the oxidative phosphorylation pathway, while genes in the module ‘green’ mainly participate in molecular functions associated with immunity like activation of T cells. Four LncRNAs (LINC00472, AL590094.1, AL365203.3, and AC147651.3) and RPL27A and RPL22L1 in the module ‘yellow’ and two lncRNAs (LINC00426 and AC129507.2) and five protein-coding genes (CSF1, NOD2, ITGAE, CD7, and PDCD1) in the module ‘green’ represented independent prognostic values in patients with ccRCC. Expression of LINC0042, NOD2, CD7, and PDCD1 were significantly correlated with ratio of immune cells (like T cells CD8 and resting mast cells). LINC00426, with significant correlation with immune cell fraction, shows potential prognostic value in ccRCC patients. Our findings provide a strategy in exploring biomarkers with prognostic significance and significant association with the fraction of immune cells.

【 授权许可】

Unknown