Influence of retinoic acid on mesenchymal stem cell differentiation in amyloid hydrogels

Data in Brief

Subhadeep Das¹  Samir K. Maji¹  Dhiman Ghosh¹  Reeba Susan Jacob¹ 

[1] Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, Maharashtra, India;

关键词: Amyloid hydrogel;    Nanofibrils;    Self-assembly;    Stem cell;    Tissue engineering;    retinoic acid;   

DOI  :  10.1016/j.dib.2015.11.015

来源: DOAJ

【 摘 要 】

This paper presents data related to the research article “Self healing hydrogels composed of amyloid nano fibrils for cell culture and stem cell differentiation” [1]. Here we probed the collective influence of all-trans retinoic acid (RA) and substrate properties (amyloid hydrogel) on human mesenchymal stem cell (hMSC) differentiation. Stem cells were cultured on soft amyloid hydrogels [1,2] in the presence and absence of matrix encapsulated RA. The cell morphology was imaged and assessed via quantification of circularity. Further immunostaining and quantitative real time PCR was used to quantify various markers of differentiation in the neuronal lineage.

【 授权许可】

Unknown