Analyses of somaclonal variation in endosperm-supported mature embryo culture of rye (Secale cereale L.)

Biotechnology & Biotechnological Equipment

Gokce Karadayi¹  Esra Arslan¹  Guleray Agar¹  Mahmut Sinan Taspinar¹  Murat Aydin¹ 

[1] Ataturk University;

关键词: Auxin;    CRED-RA;    DNA methylation;    genomic instability;    RAPD;   

DOI  :  10.1080/13102818.2016.1224980

来源: DOAJ

【 摘 要 】

Genetic variations, and especially somaclonal variations, are undesirable in genetic transformation. In this study, random amplified polymorphic DNA (RAPD) and coupled restriction enzyme digestion-random amplification (CRED-RA) markers were used for detection of the variation in calli that were obtained from endosperm-supported mature embryo of rye on Murashige and Skoog (MS) medium containing different auxins (2,4-D, dicamba and picloram) at a range of different concentrations (2, 4, 6, 8, 10 and 12 mg/L). High level of auxins caused a reduction in the genomic template stability (GTS) value. While the highest GTS was observed in the calli maintained on MS medium with 2 mg/L dicamba (98.4%), the calli maintained on MS medium with 12 mg/L picloram were found to show the least GTS (81.7%) when RAPD patterns were analysed. Epigenetic changes were more frequent and variable than genetic changes when compared to RAPD and CRED-RA results. DNA hypermethylation was observed at higher concentrations of 2,4-D and picloram, whereas DNA hypomethylation was observed in dicamba. These results indicate that RAPD and CRED-RA techniques can be used for detection of somaclonal variation in in vitro cultures, which is a fundamental step in plant genetic transformation.

【 授权许可】

Unknown