The Role of Plasma CDO1 Methylation in the Early Diagnosis of Lung Cancer

Chinese Journal of Lung Cancer

Ruifeng SHI¹  Jinghao LIU¹  Xingyu LIU¹  Qingchun ZHAO¹  Fan REN¹  Hongbing ZHANG¹  Honglin ZHAO¹  Jun CHEN¹  Pan WANG¹  Yongwen LI²  Hongyu LIU² 

[1] Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China;Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment,Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China;

关键词: cdo1;    methylation;    dna;    lung neoplasms;    plasma;   

DOI  :  10.3779/j.issn.1009-3419.2020.102.20

来源: DOAJ

【 摘 要 】

Background and objective The incidence and mortality of lung cancer often rank first in all malignant tumors. DNA methylation, as one of epigenetics, often participates in the development and progression of tumors. CDO1 as a tumor suppressor gene always undergoes methylation changes early in tumor development. Therefore, this study aims to discuss the value of CDO1 methylation in the early diagnosis of lung cancer. Methods Peripheral blood samples were collected from tumor patients and healthy people. Detection of the methylation level of CDO1 in plasma by sulfite modification and quantitative real-time PCR. Results The level of gene methylation in peripheral blood of lung cancer patients was significantly higher than that of benign lung disease patients and healthy people. The methylation level of CDO1 was significantly different in the stratified comparison of gender, lymph node metastasis and tumor-node-metastasis (TNM) stage (P<0.05). The sensitivity and specificity of CDO1 were 52.2% and 78.6%, respectively. The overall accuracy of the diagnosis was significantly higher than that of the clinical tumor markers, and the sensitivity of CDO1 to stage I and II patients was the highest (40.8%, 47.1%). In addition, CDO1 could effectively increase the sensitivity of diagnosis in multiple joint examinations. Conclusion Detecting the methylation level of CDO1 has a potentially huge advantage for the early diagnosis of lung cancer.

【 授权许可】

Unknown