Hexosamine Biosynthetic Pathway and Glycosylation Regulate Cell Migration in Melanoma Cells

Frontiers in Oncology

Isadora Araújo Oliveira¹  Adriane Regina Todeschini¹  Rafaela Muniz de Queiroz¹  Wagner Barbosa Dias¹  Adriana da Costa Pascoal¹  Bruno da Costa Rodrigues¹  Bruno Lourenço Diaz²  Bruno Piva²  Michelle Botelho Caarls³  Felipe Bouchuid Catão³ 

[1] Laboratório de Glicobiologia Estrutural e Funcional, Centro de Ciências da Saúde, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Cidade Universitária, Rio de Janeiro, Brazil;Laboratório de Inflamação, Centro de Ciências da Saúde, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Cidade Universitária, Rio de Janeiro, Brazil;Laboratório de Matriz Extracelular, Centro de Ciências da Saúde, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Cidade Universitária, Rio de Janeiro, Brazil;

关键词: hexosamine biosynthetic pathway;    O-GlcNAcylation;    glycosylation;    cell migration;    melanoma;    cancer;   

DOI  :  10.3389/fonc.2019.00116

来源: DOAJ

【 摘 要 】

The Hexosamine Biosynthetic Pathway (HBP) is a branch of glycolysis responsible for the production of a key substrate for protein glycosylation, UDP-GlcNAc. Cancer cells present altered glucose metabolism and aberrant glycosylation, pointing to alterations on HBP. Recently it was demonstrated that HBP influences many aspects of tumor biology, including the development of metastasis. In this work we characterize HBP in melanoma cells and analyze its importance to cellular processes related to the metastatic phenotype. We demonstrate that an increase in HBP flux, as well as increased O-GlcNAcylation, leads to decreased cell motility and migration in melanoma cells. In addition, inhibition of N- and O-glycosylation glycosylation reduces cell migration. High HBP flux and inhibition of N-glycosylation decrease the activity of metalloproteases 2 and 9. Our data demonstrates that modulation of HBP and different types of glycosylation impact cell migration.

【 授权许可】

Unknown