The bacterial microbiome and metabolome in caries progression and arrest

Journal of Oral Microbiology

Apoena de Aguiar Ribeiro¹  Roland R. Arnold¹  Kimon Divaris¹  Ana Paula Valente²  Ricardo Tadeu Lopes²  Tatiana K. S. Fidalgo²  Thamirys da Costa Rosa³  Bruce J. Paster⁴  Tsute Chen⁴  Hunyong Cho⁵  Liana B. Freitas-Fernandes⁶  Aline de Almeida Neves⁶  Di Wu⁷  Kevin Moss⁷  M. Andrea Azcarate-Peril⁸ 

[1] Adams School of Dentistry, University of North Carolina;Federal University of Rio de Janeiro;Fluminense Federal University;Forsyth Institute;Gillings School of Global Public Health, University of North Carolina;Rio de Janeiro Federal University;School of Dentistry, University of North Carolina;University of North Carolina School of Medicine;

关键词: dental caries;    microct;    oral bacterial microbiome;    metabolome;    bacteria;   

DOI  :  10.1080/20002297.2021.1886748

来源: DOAJ

【 摘 要 】

Aim: This in vivo experimental study investigated bacterial microbiome and metabolome longitudinal changes associated with enamel caries lesion progression and arrest. Methods: We induced natural caries activity in three caries-free volunteers prior to four premolar extractions for orthodontic reasons. The experimental model included placement of a modified orthodontic band on smooth surfaces and a mesh on occlusal surfaces. We applied the caries-inducing protocol for 4- and 6-weeks, and subsequently promoted caries lesion arrest via a 2-week toothbrushing period. Lesions were verified clinically and quantitated via micro-CT enamel density measurements. The biofilm microbial composition was determined via 16S rRNA gene Illumina sequencing and NMR spectrometry was used for metabolomics. Results: Biofilm maturation and caries lesion progression were characterized by an increase in Gram-negative anaerobes, including Veillonella and Prevotella. Streptococcus was associated caries lesion progression, while a more equal distribution of Streptococcus, Bifidobacterium, Atopobium, Prevotella, Veillonella, and Saccharibacteria (TM7) characterized arrest. Lactate, acetate, pyruvate, alanine, valine, and sugars were more abundant in mature biofilms compared to newly formed biofilms. Conclusions: These longitudinal bacterial microbiome and metabolome results provide novel mechanistic insights into the role of the biofilm in caries progression and arrest and offer promising candidate biomarkers for validation in future studies.

【 授权许可】

Unknown