Generation of Vestibular Tissue-Like Organoids From Human Pluripotent Stem Cells Using the Rotary Cell Culture System

Frontiers in Cell and Developmental Biology

Babak Nasr¹  Cristiana Mattei³  Kathryn S. Stok³  Giovanna M. D'Abaco³  Zihui Li³  Bryony A. Nayagam⁵  Mirella Dottori⁶  Hannah Drury⁷  Rebecca Lim⁷  Melissa A. Tadros⁷ 

[1] ARC Centre of Excellence for Integrative Brain Function, The University of Melbourne, Melbourne, VIC, Australia;Centre for Neural Engineering, Melbourne School of Engineering, The University of Melbourne, Melbourne, VIC, Australia;Department of Biomedical Engineering, Melbourne School of Engineering, The University of Melbourne, Melbourne, VIC, Australia;Department of Electrical and Electronic Engineering, Melbourne School of Engineering, The University of Melbourne, Melbourne, VIC, Australia;Departments of Audiology and Speech Pathology and Department of Medical Bionics, The University of Melbourne, Melbourne, VIC, Australia;Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW, Australia;School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle, Newcastle, NSW, Australia;

关键词: vestibular hair cells;    human pluripotent stem cells;    organoids;    human fetal tissue;    inner ear;   

DOI  :  10.3389/fcell.2019.00025

来源: DOAJ

【 摘 要 】

Hair cells are specialized mechanosensitive cells responsible for mediating balance and hearing within the inner ear. In mammals, hair cells are limited in number and do not regenerate. Human pluripotent stem cells (hPSCs) provide a valuable source for deriving human hair cells to study their development and design therapies to treat and/or prevent their degeneration. In this study we used a dynamic 3D Rotary Cell Culture System (RCCS) for deriving inner ear organoids from hPSCs. We show RCCS-derived organoids recapitulate stages of inner ear development and give rise to an enriched population of hair cells displaying vestibular-like morphological and physiological phenotypes, which resemble developing human fetal inner ear hair cells as well as the presence of accessory otoconia-like structures. These results show that hPSC-derived organoids can generate complex inner ear structural features and be a resource to study inner ear development.

【 授权许可】

Unknown