Design of indirect ELISA-based test-system for determination of Mannheimia haemolytica antibody titre in bovine sera

Ветеринария сегодня

O. V. Pruntova¹  O. P. Bjadovskaya¹  Ye. V. Peshkova¹  Ye. S. Kostrova¹ 

[1] ФГБУ «ВНИИЗЖ», г. Владимир;

关键词: mannheimia haemolytica;    иммуноферментный анализ;    липополисахаридный антиген;    крупный рогатый скот;    mannheimia haemolytica;    enzyme-linked immunosorbent assay;    lipopolysaccharide antigen;    cattle;   

DOI  :  

来源: DOAJ

【 摘 要 】

Indirect ELISA-based test-system was developed for determination of Mannheimia haemolytica antibody titre in sera from cattle of different age-sex groups. Lipopolysaccha-ride antigen (LPS-antigen) produced by phenol-water extraction from Mannheimia haemolytica strain No. 29696 (ATCC collection) was used as an antigen. Results obtained during the comparative examination of bovine sera using the proposed test-system and commercial test-system «Mannheimia haemolytica ELISA KIT» (BIOX, Belgium) are demonstrated. Relative specificity and sensitivity of the designed method were determined.

【 授权许可】

Unknown