Ветеринария сегодня | |
Design of indirect ELISA-based test-system for determination of Mannheimia haemolytica antibody titre in bovine sera | |
O. V. Pruntova1  O. P. Bjadovskaya1  Ye. V. Peshkova1  Ye. S. Kostrova1  | |
[1] ФГБУ «ВНИИЗЖ», г. Владимир; | |
关键词: mannheimia haemolytica; иммуноферментный анализ; липополисахаридный антиген; крупный рогатый скот; mannheimia haemolytica; enzyme-linked immunosorbent assay; lipopolysaccharide antigen; cattle; | |
DOI : | |
来源: DOAJ |
【 摘 要 】
Indirect ELISA-based test-system was developed for determination of Mannheimia haemolytica antibody titre in sera from cattle of different age-sex groups. Lipopolysaccha-ride antigen (LPS-antigen) produced by phenol-water extraction from Mannheimia haemolytica strain No. 29696 (ATCC collection) was used as an antigen. Results obtained during the comparative examination of bovine sera using the proposed test-system and commercial test-system «Mannheimia haemolytica ELISA KIT» (BIOX, Belgium) are demonstrated. Relative specificity and sensitivity of the designed method were determined.
【 授权许可】
Unknown