Identification of mycobacterium tuberculosis beijing genotype using three different molecular methods

Majallah-i ̒Ilmī-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Simnān

关键词: Spoligotyping;    VNTR typing;    RFLP;    Mycobacterium tuberculosis;    Beijing genotype;   

DOI  :  

来源: DOAJ

【 摘 要 】

Introduction: Beijing strains constitute more than 1/4 of Mycobacterium tuberculosis (MT) genotypes.Beijing genotype is considered an important genotype because of its reasonable characteristics such asassociation with multi-drugs resistance TB. The aim of this study was to identify the genetic pattern ofMT Beijing genotype using spoligotyping, variable number tandem repeat (VNTR) typing and RFLP-IS6110methods.Materials ands Methods: 238 MT culture positive specimens (2007-2008) were genotyped byspoligotyping. Thereafter, the isolated Beijing genotype was subjected to VNTR typing and RFLPanalysis. The results of Spoligotyping were analyzed using SPOLDB4 database. VNTR typing was usedto identify alleles diversity in 9 locus (MPTR-A, ETR-A, ETR-B, ETR-C, ETR-D, ETR-E, ETR-F,QUB11B, QUB3232) of the isolated Beijing strains. The allelic diversity of VNTR was measured usingHunter Gaston Index (HGI).Results: The spoligotyping of MT isolates revealed the following 8 patterns: Haarlem (27.7%), CAS1(25.2%), EAI3 (21.8%), CAS2 (6.7%), T1 (6.3%), Beijing (5.5%), U (5%), T (0.4), EAI2 (1.2%). ByVNTR typing, the QUB3232 locus was identified as the most distinctive (HGI≥0.6); ETR-F, ETR-E andQUB11b loci; as median distinctive loci (HGI≥0.4-0.6) and other loci as the weakest distinctive locus forBeijing families.The Beijing strains demonstrated diverse patterns in RFLP, 13/13(100%) and VNTR10/13(77%).Conclusion: The ariety of genetic patterns revealed the reactivation in the studied population. AsRFLP method is a time consuming and costly effective method, VNTR and spoligotyping method can beused as alternative methods for molecular epidemiology of MT.

【 授权许可】

Unknown