Complex Toxin Profile of French MediterraneanOstreopsis cf. ovata Strains, Seafood Accumulation and Ovatoxins Prepurification

Marine Drugs

Nicolas Chomérat¹  Charline Brissard²  Fabienne Hervé²  Francesco Pisapia²  Véronique Séchet²  Philipp Hess²  Jocelyn Harcouet²  Zouher Amzil²  Rodolphe Lémée³  Christine Herrenknecht⁴ 

[1] Ifremer, Laboratoire Environnement Ressource de Bretagne Occitentale (LER-BO), Marine Biological Station, BP 40537, Concarneau F-29185, France;Ifremer, Phycotoxins Laboratory, rue de l'Ile d'Yeu, BP 21105, Nantes F-44311, France;Sorbonne Universités, UPMC Univ Paris 06, UMR 7093, LOV, Observatoire Océanologique, Villefranche/mer F-06230, France;Université Nantes Angers Le Mans (LUNAM), University of Nantes, MMS EA2160, Pharmacy Faculty, 9 rue Bias, Nantes F-44035, France;

关键词: Ostreopsis cf. ovata;    palytoxin &;    ovatoxins;    culture;    seafood contamination;    LC-MS/MS;    hemolysis assay;    Mediterranean Sea;   

DOI  :  10.3390/md12052851

来源: DOAJ

【 摘 要 】

Ostreopsis cf. ovata produces palytoxin analogues including ovatoxins (OVTXs) and a putative palytoxin (p-PLTX), which can accumulate in marine organisms and may possibly lead to food intoxication. However, purified ovatoxins are not widely available and their toxicities are still unknown. The aim of this study was to improve understanding of the ecophysiology of Ostreopsis cf. ovata and its toxin production as well as to optimize the purification process for ovatoxin. During Ostreopsis blooms in 2011 and 2012 in Villefranche-sur-Mer (France, NW Mediterranean Sea), microalgae epiphytic cells and marine organisms were collected and analyzed both by LC-MS/MS and hemolysis assay. Results obtained with these two methods were comparable, suggesting ovatoxins have hemolytic properties. An average of 223 μg·kg−1 of palytoxin equivalent of whole flesh was found, thus exceeding the threshold of 30 μg·kg−1 in shellfish recommended by the European Food Safety Authority (EFSA). Ostreopsis cells showed the same toxin profile both in situ and in laboratory culture, with ovatoxin-a (OVTX-a) being the most abundant analogue (~50%), followed by OVTX-b (~15%), p-PLTX (12%), OVTX-d (8%), OVTX-c (5%) and OVTX-e (4%). Ostreopsis cf. ovata produced up to 2 g of biomassper L of culture, with a maximum concentration of 300 pg PLTX equivalent cell−1. Thus, an approximate amount of 10 mg of PLTX-group toxins may be produced with 10 L of this strain. Toxin extracts obtained from collected biomass were purified using different techniques such as liquid-liquid partition or size exclusion. Among these methods,open-column chromatography with Sephadex LH20 phase yielded the best results with a cleanup efficiency of 93% and recovery of about 85%, representing an increase of toxin percentage by 13 fold. Hence, this purification step should be incorporated into future isolation exercises.

【 授权许可】

Unknown