Generation and live imaging of tumors with specific genotypes in the living fly pupa

STAR Protocols

Zsuzsa Markus¹  Emma Szamek²  Brenda Canales Coutiño³  Marios Georgiou³ 

[1] Corresponding author;Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK;School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK;

关键词: Cell Biology;    Cancer;    Genetics;    Microscopy;    Model Organisms;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: This protocol describes the step-by-step generation of tumors with specific genotypes on the dorsal thorax epithelium of the fly. This in vivo system allows the imaging of tumor cell morphology and behavior in high spatial and temporal resolution. Phenotypes such as cell invasion, cell division, and tumor size can be quantified and compared to specific controls or to the neighboring wild-type tissue. Thus, this model allows the study of conserved genes that enhance or suppress epithelial tumor progression.For complete details on the use and execution of this protocol, please refer to Canales Coutiño et al. (2020).

【 授权许可】

Unknown