Comprehensive RNA-seq reveals molecular changes in kidney malignancy among people living with HIV

Molecular Therapy: Nucleic Acids

Jingjing Xu¹  Qiuyue Li²  Jianqing Ye³  Xiaoyan Zhang⁴  Christopher Corpe⁵  Xiaomeng He⁵  Zehuan Li⁵  Jianqing Xu⁵  Heng Zou⁵  Juan Bao⁵  Shanshan Liu⁵  Tongyu Zhu⁵  Jin Wang⁵  Feng Liu⁵  Lin Wang⁵  Yanling Feng⁵ 

[1] Department of Urology, The Third Affiliated Hospital of Second Military Medical University, Shanghai, China;Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, China;Department of Urology, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, 1665 Kongjiang Road, Shanghai, China;King’s College London, London, Nutritional Science Department, 150 Stamford Street, Waterloo, London SE19NH, UK;Shanghai Public Health Clinical Center, Fudan University, 2901 Caolang Road, Jinshan District, Shanghai 201508, China;

关键词: kidney malignancy;    HIV infection;    biomarker;    RNA-seq;    HIV-associated kidney cancer;    KISS1R;   

DOI  :  

来源: DOAJ

【 摘 要 】

To heighten the awareness of kidney malignancy in patients with HIV infection to facilitate the early diagnosis of kidney cancer, the differentially expressed mRNAs were analyzed in this malignant tumor using RNA sequencing. We identified 2,962 protein-coding transcripts in HIV-associated kidney cancer. KISS1R, CAIX, and NPTX2 mRNA expression levels were specifically increased in HIV-associated kidney cancer while UMOD and TMEM213 mRNA were decreased in most cases based on real-time PCR analyses. These findings were similar to those noted for the general population with renal cell carcinoma. Immunohistochemical staining analysis also showed that a total of 18 malignant kidney cases among the people living with HIV (PLWH) exhibited positive staining for KISS1R and CAIX. Pathway analysis of the differentially expressed mRNAs in HIV-associated kidney cancer revealed that several key pathways were involved, including vascular endothelial growth factor-activated receptor activity, IgG binding, and lipopolysaccharide receptor activity. Altogether, our findings reveal the identified molecular changes in kidney malignancy, which may offer a helpful explanation for cancer progression and open up new therapeutic avenues that may decrease mortality after a cancer diagnosis among PLWH.

【 授权许可】

Unknown