AFM and MFM techniques for enzyme activity imaging and quantification

Biotechnology & Biotechnological Equipment

Israel Morales-Reyes¹  Nikola Batina¹  Michelle Arredondo²  Margarita Stoytcheva³ 

[1] División de Ciencias Básicas e Ingeniería;Tecnológico Nacional de México;Universidad Autónoma de Baja California;

关键词: Atomic force microscopy;    magnetic force microscopy;    trypsin activity;    ferrogel;   

DOI  :  10.1080/13102818.2018.1470904

来源: DOAJ

【 摘 要 】

In this work, a new approach for enzyme activity monitoring is suggested. It is based on the real-time imaging, by atomic force microscopy (AFM) and magnetic force microscopy (MFM), of the degradation of a nanoparticles-loaded enzyme responsive layer, namely Fe3O4-nanoparticles-loaded gelatin. The roughness analysis allowed the quantification of the trypsin-induced ferrogel degradation and the correlation of the AFM and MFM data obtained.

【 授权许可】

Unknown