Molecular Docking of Multidrug ResistantKlebsiella pneumoniae from River Water ofKlang Valley, Malaysia

Journal of Pure and Applied Microbiology

Gnanendra Shanmugam¹  Balavinayagamani Ganapathy²  Sridevi Subramonie²  Yoke Ing Kwan²  Nagaraja Suryadevara²  Ponmurugan P.³  Venkatasathya Sai Appala Raju Velaga⁴ 

[1] 2Department of Biotechnology, College of Life and Applied Sciences, Yeungnam University, Gyeongsan, Gyeongbuk, 38541, Korea. ;1Department of Biomedical Sciences, Faculty of Medicine, MAHSA University, Selangor, Malaysia. ;3Biomedical Research Lab, Department of Botany, Bharathiar University, Coimbatore - 641 046, Tamil Nadu, India. ;4Department of Medicinal Chemistry, Faculty of Pharmacy, MAHSA University, Malaysia.;

关键词: k. pneumoniae;    mdr;    esbl;    blatem;    blactx-m;   

DOI  :  10.22207/JPAM.13.4.26

来源: DOAJ

【 摘 要 】

Malaysia Klang River covering states of Selangor and Kuala Lumpur, has been severely polluted from urbanization, industrialization and increased hospitals. By serving as reservoir for bacterial gene transformation, Multidrug Resistant (MDR) strains especially Extended Spectrum Beta Lactamase (ESBL) resistant strains of Klebsiella pneumoniae (K. pneumoniae) have increased. To identify MDR K.pneumoniae; phenotypically determine ESBL resistant traits and molecular characterize these strains, 50 water samples were collected along Klang River and their physicochemical properties were determined. Gram-negative K. pneumoniae isolates were tested for multidrug and ESBL resistance using Kirby-Bauer method. DNA of ESBL resistant K. pneumoniae were extracted and amplified using Polymerase Chain Reaction (PCR); and conferred using blaTEM and blaCTX-M genes. Through β-lactamase gene sequencing and docking studies, the effectiveness of drugs against ESBL resistant K.pneumoniae were determined. A total of 31 (62.0%) K.pneumoniae were isolated with 12 (38.7%) positive MDR strains and 5 (41.7%) ESBL resistant strains. Only 1 (20%) blaTEM gene and 4 (80%) blaCTX-M genes were detected with Ceftriaxone (CRO) as the most effective drug showing highest binding energy (-9.2Kcal/mol) against ESBL resistant K. pneumoniae. With high prevalence of CTX-M-type ESBL resistant K.pneumoniae in Klang River, effective environmental and antibiotic controls should be adopted.

【 授权许可】

Unknown