Area light source‐triggered latent angiogenic molecular mechanisms intensify therapeutic efficacy of adult stem cells

Bioengineering & Translational Medicine

Sung Young Park¹  Haeshin Lee²  Hye Ran Jeon³  Dong‐Ik Kim⁴  Gun‐Jae Jeong⁴  Sung Min Cho⁵  Sung‐Won Kim⁵  Suk Ho Bhang⁵  Gwang‐Bum Im⁵  Yu‐Jin Kim⁵  Yeong Hwan Kim⁵ 

[1] Department of Chemical and Biological Engineering Korea National University of Transportation Chungju Republic of Korea;Department of Chemistry, Center for Nature‐Inspired Technology (CNiT) Korea Advanced Institute of Science and Technology (KAIST) Daejeon Republic of Korea;Department of Health Sciences and Technology, SAIHST Sungkyunkwan University Seoul Republic of Korea;Division of Vascular Surgery, Samsung Medical Center Sungkyunkwan University School of Medicine Seoul Republic of Korea;School of Chemical Engineering Sungkyunkwan University Suwon Republic of Korea;

关键词: human adipose‐derived stem cells;    ischemic diseases;    latent reaction period;    red organic light‐emitting diode;    therapeutic angiogenesis;   

DOI  :  10.1002/btm2.10255

来源: DOAJ

【 摘 要 】

Abstract Light‐based therapy such as photobiomodulation (PBM) reportedly produces beneficial physiological effects in cells and tissues. However, most reports have focused on the immediate and instant effects of light. Considering the physiological effects of natural light exposure in living organisms, the latent reaction period after irradiation should be deliberated. In contrast to previous reports, we examined the latent reaction period after light exposure with optimized irradiating parameters and validated novel therapeutic molecular mechanisms for the first time. we demonstrated an organic light‐emitting diode (OLED)‐based PBM (OPBM) strategy that enhances the angiogenic efficacy of human adipose‐derived stem cells (hADSCs) via direct irradiation with red OLEDs of optimized wavelength, voltage, current, luminance, and duration, and investigated the underlying molecular mechanisms. Our results revealed that the angiogenic paracrine effect, viability, and adhesion of hADSCs were significantly intensified by our OPBM strategy. Following OPBM treatment, significant changes were observed in HIF‐1α expression, intracellular reactive oxygen species levels, activation of the receptor tyrosine kinase, and glycolytic pathways in hADSCs. In addition, transplantation of OLED‐irradiated hADSCs resulted in significantly enhanced limb salvage ratio in a mouse model of hindlimb ischemia. Our OPBM might serve as a new paradigm for stem cell culture systems to develop cell‐based therapies in the future.

【 授权许可】

Unknown