Identification of an Integrase That Responsible for Precise Integration and Excision of Riemerella anatipestifer Genomic Island

Frontiers in Microbiology

Yuncai Xiao¹  Deshi Shi¹  Dingren Bi¹  Shaowen Li¹  Xianrong Meng¹  Zili Li¹  Zutao Zhou¹  Mei Liu¹  Sishun Hu¹  Yijie Cui³  Zhijian Sun³  Yang Zhang³  Ying Wang³ 

[1] Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture of the People’s Republic of China, Wuhan, China;Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, China;State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China;

关键词: Riemerella anatipestifer;    genomic island;    integrase;    integration;    excision;    gene expression;   

DOI  :  10.3389/fmicb.2019.02099

来源: DOAJ

【 摘 要 】

Riemerella anatipestifer is a Gram-negative, pathogenic bacterium, which is harmful to poultry. However, the genomic islands (GIs) in R. anatipestifer are not well-studied. In this study, a 10K genomic island was predicted by the bioinformatics analysis of R. anatipestifer ATCC 11845, which is widely found in other R. anatipestifer genomes. We had first reported the genomic island integration and excision function in R. anatipestifer. We successfully constructed the integration plasmid by using the integrase and 53 bp attP elements. The 10K GI was found integrated at the 53 bp attB located in the Arg-tRNA of the R. anatipestifer RA-YM chromosome. We identified an integrase that helped in the precise integration and excision in R. anatipestifer and elucidated the molecular mechanism of the 10K genomic island integration and excision. Furthermore, we provided a new method for the gene expression and construction of complementary strain.

【 授权许可】

Unknown