Standardization of a protocol to extract and analyze chlorophyll a and carotenoids in Gracilaria tenuistipitata Var. Liui. Zhang and Xia (Rhodophyta)

Brazilian Journal of Oceanography

Adriana Mercadante¹  Fernanda Mandelli¹  Cláudia Maria Furlan¹  Fungyi Chow²  Priscila Bezerra Torres²  Déborah Yara Alves Cursino dos Santos² 

[1] Universidade Estadual de Campinas;Universidade de São Paulo;

关键词: Gracilaria tenuistipitata;    Clorofila a;    Carotenoides;    Espectrometria UV-visível;    CLAE;   

DOI  :  10.1590/s1679-87592014068106201

来源: DOAJ

【 摘 要 】

Chlorophyll a and carotenoids are important pigments in photosynthesis. Several studies have been published describing extraction and analysis protocols of these pigments, mainly in vascular plant species. This study standardizes an extraction and analysis protocol of these substances in Gracilaria tenuistipitata var. liui, a red seaweed. Apical portions grown in vitro were triturated in liquid nitrogen. Extracts were prepared in 1.5 mL solvent and centrifuged. Quantitative and qualitative analyses of pigments were performed by UV/visible light spectrophotometry and high performance liquid chromatography (HPLC) and HPLC coupled to mass spectrometry (HPLC-MS). The parameters assessed were: minimum biomass, best extraction solvent, and number of extraction steps. Methanol was the most efficient solvent, and 50 mg fresh biomass was the amount of sample indicated, submitted to one single extraction step. No significant differences were observed in levels of these pigments by UV-visible light spectrophotometry and HPLC. However, HPLC or HPLC-MS are required to identify the different carotenoids present in this seaweed species.

【 授权许可】

Unknown