Molecular regulation of high muscle mass in developing Blonde d'Aquitaine cattle foetuses

Biology Open

Antonio Reverter¹  Nicholas J. Hudson²  Brigitte Picard³  Isabelle Cassar-Malek³  Céline Boby³ 

[1] Agriculture, Commonwealth Science and Industrial Research Organisation, Queensland Bioscience Precinct, St. Lucia, Brisbane 4075, Australia;School of Agriculture and Food Sciences, University of Queensland, Brisbane 4075, Australia;UMR1213 Herbivores, Institut National de la Recherche Agronomique, VetAgro Sup, 63122 Saint Genès Champanelle, Clermont-Ferrand F-63122, France;

关键词: Skeletal muscle;    Myostatin;    Mitochondria;    Genomics;    Myogenesis;   

DOI  :  10.1242/bio.024950

来源: DOAJ

【 摘 要 】

The Blonde d'Aquitaine (BA) is a French cattle breed with enhanced muscularity, partly attributable to a MSTN mutation. The BA m. Semitendinosus has a faster muscle fibre isoform phenotype comprising a higher proportion of fast type IIX fibres compared to age-matched Charolais (CH). To better understand the molecular network of modifications in BA compared to CH muscle, we assayed the transcriptomes of the m. Semitendinosus at 110, 180, 210 and 260 days postconception (dpc). We used a combination of differential expression (DE) and regulatory impact factors (RIF) to compare and contrast muscle gene expression between the breeds. Prominently developmentally regulated genes in both breeds reflected the replacement of embryonic myosin isoforms (MYL4, MYH3) with adult isoforms (MYH1) and the upregulation of mitochondrial metabolism (CKMT2, AGXT2L1) in preparation for birth. However, the transition to a fast, glycolytic muscle phenotype in the MSTN mutant BA is detectable through downregulation of various slow twitch subunits (TNNC1, MYH7, TPM3, CSRP3) beyond 210 dpc, and a small but consistent genome-wide reduction in mRNA encoding the mitoproteome. Across the breeds, NRIP2 is the regulatory gene possessing a network change most similar to that of MSTN.

【 授权许可】

Unknown