Effect of Various Compounds Blocking the Colony Pigmentation on the Aflatoxin B1 Production by Aspergillus flavus

Toxins

Lyudmila A. Limantseva¹  Vitaly G. Dzhavakhiya¹  Natalia V. Statsyuk¹  Larisa A. Shcherbakova¹  Sofya B. Popletaeva¹  Tatiana M. Voinova¹ 

[1] All-Russian Research Institute of Phytopathology, Bolshie Vyazemy, Moscow 143050, Russia;

关键词: aflatoxin B1;    fungal melanins;    polyketide biosynthesis;    compactin;    thymol;    fluconazole;    3-hydroxybenzaldehyde;    Aspergillus flavus;   

DOI  :  10.3390/toxins8110313

来源: DOAJ

【 摘 要 】

Aflatoxins and melanins are the products of a polyketide biosynthesis. In this study, the search of potential inhibitors of the aflatoxin B1 (AFB1) biosynthesis was performed among compounds blocking the pigmentation in fungi. Four compounds—three natural (thymol, 3-hydroxybenzaldehyde, compactin) and one synthetic (fluconazole)—were examined for their ability to block the pigmentation and AFB1 production in Aspergillus flavus. All compounds inhibited the mycelium pigmentation of a fungus growing on solid medium. At the same time, thymol, fluconazole, and 3-hydroxybenzaldehyde stimulated AFB1 accumulation in culture broth of A. flavus under submerged fermentation, whereas the addition of 2.5 μg/mL of compactin resulted in a 50× reduction in AFB1 production. Moreover, compactin also suppressed the sporulation of A. flavus on solid medium. In vivo treatment of corn and wheat grain with compactin (50 μg/g of grain) reduced the level of AFB1 accumulation 14 and 15 times, respectively. Further prospects of the compactin study as potential AFB1 inhibitor are discussed.

【 授权许可】

Unknown