Ветеринария сегодня | |
PURITY TESTING OF ARRIAH FMD VACCINES | |
V. A. Starikov1  A. S. Sharypov1  M. I. Doronin1  D. V. Mikhalishin1  D. A. Lozovoy1  | |
[1] ФГБУ «ВНИИЗЖ», г. Владимир; | |
关键词: антитела; неструктурные белки вируса ящура; противоящурная вакцина; клеточный детрит; иммуноферментный анализ; antibodies; fmdv non-structural proteins; fmd vaccine; cell debris; elisa; | |
DOI : | |
来源: DOAJ |
【 摘 要 】
The paper demonstrates results of FMD vaccine testing for their inability to induce antibodies to FMD virus non-structural proteins (FMDV NSP antibodies) in cattle at different dates post vaccination. Subtype A, O, Asia-1 and SAT-2 cultural FMD virus replicated in baby hamster kidney suspension culture (ВНК-21/2-17) was used for vaccine production. FMDV antigen was inactivated with aminoethyl ethylenimine solution, and the suspension was subsequently purified. Non NSP-purified preparation was made to simulate presence of NSPs in the vaccine. ELISA test-kits were used for testing cattle sera collected before and after immunization for presence of 3ABC polyprotein-specific antibodies. Inoculation of cattle with non-purified preparation induced antibodies specific to FMDV NSPs in 87.5% animals on day 30, in 75% animals on day 44 and in 62.5% animals on day 132 post third immunization. All tested vaccines manufactured by the FGBI “ARRIAH” were demonstrated to induce no FMDV NSP antibodies suggesting their complete removal from the virus-containing suspension.
【 授权许可】
Unknown