PURITY TESTING OF ARRIAH FMD VACCINES

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V. A. Starikov¹  A. S. Sharypov¹  M. I. Doronin¹  D. V. Mikhalishin¹  D. A. Lozovoy¹ 

[1] ФГБУ «ВНИИЗЖ», г. Владимир;

关键词: антитела;    неструктурные белки вируса ящура;    противоящурная вакцина;    клеточный детрит;    иммуноферментный анализ;    antibodies;    fmdv non-structural proteins;    fmd vaccine;    cell debris;    elisa;   

DOI  :  

来源: DOAJ

【 摘 要 】

The paper demonstrates results of FMD vaccine testing for their inability to induce antibodies to FMD virus non-structural proteins (FMDV NSP antibodies) in cattle at different dates post vaccination. Subtype A, O, Asia-1 and SAT-2 cultural FMD virus replicated in baby hamster kidney suspension culture (ВНК-21/2-17) was used for vaccine production. FMDV antigen was inactivated with aminoethyl ethylenimine solution, and the suspension was subsequently purified. Non NSP-purified preparation was made to simulate presence of NSPs in the vaccine. ELISA test-kits were used for testing cattle sera collected before and after immunization for presence of 3ABC polyprotein-specific antibodies. Inoculation of cattle with non-purified preparation induced antibodies specific to FMDV NSPs in 87.5% animals on day 30, in 75% animals on day 44 and in 62.5% animals on day 132 post third immunization. All tested vaccines manufactured by the FGBI “ARRIAH” were demonstrated to induce no FMDV NSP antibodies suggesting their complete removal from the virus-containing suspension.

【 授权许可】

Unknown