Conventional PCR primers for the detection of grapevine pathogens disseminated by propagating material

International Journal of Horticultural Science

R. Lózsa¹  I. Ember¹  T. Dula²  N. Czotter³  É. Várallyay³  E. Manduláné Farkas⁴  E. Szegedi⁴ 

[1] Corvinus University of Budapest, Faculty of Horticulture, Department of Viticulture, 1118 Budapest, Villányi út 29-43., Hungary;DULA Grape & Wine Advisory Ltd., 3300 Eger, Eszterházy tér 9.;National Agricultural Research and Innovation Centre, Agricultural Biotechnology Institute, 2100 Gödöllô, Szent-Györgyi Albert út 4., Hungary;National Agricultural Research and Innovation Centre, Research Institute for Viticulture and Enology, Experimental Station of Kecskemét, 6000 Kecskemét-Katonatelep, Katona Zsigmond út 5., Hungary;

关键词: bacteria;    fungi;    phytoplasmas;    polymerase chain reaction;    primer sequences;    viroids;   

DOI  :  10.31421/IJHS/20/3-4/1139

来源: DOAJ

【 摘 要 】

Polymerase chain reaction driven by sequence specific primers has become the most widely used diagnostic method to detect and identify plant pathogens. The sensitive and cost-effective pathogen detection is exceptionally important in the production of propagating material. In this paper we have collected primer sequence data from the literature for the detection of the most important grapevine pathogens disseminated by propagating stocks by conventional polymerase chain reaction. Basic protocols to obtain template nucleic acids have also been briefly rewieved.

【 授权许可】

Unknown