Cadmium-Binding Polypeptides in Cadmium-Treated Zinnia

Taiwania

Yung-Reui Chen¹  Shieu-Yun Ma¹  Thing-Chien Tsay¹ 

[1] Department of Botany, National Taiwan University, Taipei, Taiwan.;

关键词: Cadmium;    Glutathione;    Phytochelatins;    Sulfurhydryl group;    Zinnia elegans;   

DOI  :  10.6165/tai.2002.47(1).11

来源: DOAJ

【 摘 要 】

Five-week-old seedlings of Zinnia elegans were hydroponically cultured in Hoagland's solution containing 45 µM Cd2+ with or without one week of prior exposure to 10 µM Cd2+. Profiles of HPLC indicated that plants previously exposed to 10 µM Cd2+ developed more sulfurhydryl groups and glutathione than plants not exposed to that prior treatment. Individual peaks of sulfurhydryl groups displayed a prominent increase at 24 h, following by a gradual decrease to initial levels of sulfurhydryl after 96 h. Further isolation and purification of metal-binding complexes was done using DE 52, Sephadex, and HPLC chromatographic separations. Two fractions of the LMW complex contained more than 90% of the histidine-like substance in total amino acids. The HMW complex had five peaks in the HPLC profile. One major peak contained more than 85% of the histidine-like substance, whereas other peaks contained less than 0.1% of the histidine, along with phytochelatins with n number 3 and 4.

【 授权许可】

Unknown