Endometrial TGF-β, IL-10, IL-17 and autophagy are dysregulated in women with recurrent implantation failure with chronic endometritis

Reproductive Biology and Endocrinology

Fu-jun Liu¹  Hong Zhang²  Zhong-quan Chen³  Wei Zhang⁴  Wen-juan Wang⁴  Jiang-ye Fang⁴  Xue-mei Liu⁴  Joanne Kwak-Kim⁵ 

[1] Central Laboratory, Yantai Yuhuangding Hospital of Qingdao University;Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Soochow University;Department of anatomy, Binzhou Medical University;Reproduction Medical Center, Yantai Yuhuangding Hospital of Qingdao University;Reproductive Medicine and Immunology, Department of Obstetrics and Gynecology, Chicago Medical School, Rosalind Franklin University of Medicine and Science;

关键词: Repeated implantation failure;    TGF-β;    IL-10;    IL-17;    Chronic endometritis;    Autophagy;   

DOI  :  10.1186/s12958-018-0444-9

来源: DOAJ

【 摘 要 】

Abstract Background Chronic endometritis (CE) is a condition which results in reduced receptivity of embryos by dysregulated lymphocyte subsets, abnormal expression of cytokines, chemokines and other regulatory molecules in the endometrium (EM). Macroautophagy (autophagy), the highly conserved cellular homeostasis pathway, plays an essential role in the development and function of T lymphocytes, and supports T cell lineage stability and survival fitness. The possible relationships between autophagy and local cytokine milieus in repeated implantation failure (RIF) with CE have not been elucidated yet. Methods This case-control study was performed at a large reproductive medicine center between February 2015 and July 2016. Seventy-five recurrent implantation falliure women with CE who had “strawberry aspect” and 75 women with male factor infertility were included. In this study, endometrial expressions of IL-17, IL-10, TGF-β and autophagy related molecules, including LC3-II and mTORC1 were investigated by qRT-PCR, Western blot, immunofluorescence and immunohistochemistry assays. Results The expression of IL-17 was significantly higher in patients with CE compared to women with male factor infertility, while the expressions of IL-10 and TGF-β were significantly lower. Moreover, the expression of autophagy (LC3-II) is increased, while the expression of mTORC1 was impaired. Conclusions CE is associated with shifted cytokine milieu towards Th17 over Treg immunity in endometrium through impaired autophagy by decreased mTORC1.

【 授权许可】

Unknown