Surface Modulation of Graphene Oxide for Amidase Immobilization with High Loadings for Efficient Biocatalysis

Biomolecules

Kongliang Xu¹  Renchao Zheng¹  Bin Wang¹  Chenlu Si¹  Chaoping Lin¹  Yuguo Zheng¹ 

[1] Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China;

关键词: amidase;    enzyme immobilization;    graphene oxide;    high loading;    biocatalysis;    surface modulation;   

DOI  :  10.3390/biom11101399

来源: DOAJ

【 摘 要 】

As a type of important and versatile biocatalyst, amidase immobilization on solid materials has received broad attention with its relatively easy procedure and available reusability. However, current porous supports have suffered from limited loadings, and it is highly desired to develop a new type of material with abundant space so as to ensure a high loading of amidase. Here, graphene oxide was adopted as the support for amidase immobilization, which showed the highest loading capacity for amidase (~3000 mg/g) to date. To the best of our knowledge, it is the first case of amidase immobilized on graphene oxide. Through surface modulation via reducing the contents of oxygen-containing functional groups, activity recovery of immobilized amidase increased from 67.8% to 85.3%. Moreover, surface-modulated graphene oxide can efficiently uptake amidase under a wide range of pH, and the maximum loading can reach ~3500 mg/g. The resultant biocomposites exhibit efficient biocatalytic performance for asymmetric synthesis of a chiral amino acid (i.e., L-4-fluorophenylglycine, an intermediate of aprepitant).

【 授权许可】

Unknown