High Hydrostatic Pressure Inducible Trimethylamine N-Oxide Reductase Improves the Pressure Tolerance of Piezosensitive Bacteria Vibrio fluvialis

Frontiers in Microbiology

Xue-Gong Li¹  Wei-Jia Zhang¹  Hao Zhou²  Qun-Jian Yin³  Xiao-Qing Qi³  Claire-Lise Santini⁴  Long-Fei Wu⁴  I-Ming Chou⁵  Ying Chen⁵  Sheng-Da Zhang⁶  Ting Jiang⁷ 

[1] CAS Key Laboratory for Experimental Study under Deep-sea Extreme Conditions, Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya, China;Engineering Laboratory of Engineering Department, Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya, China;International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms, CNRS-Marseille/CAS, Beijing, China;LCB UMR 7283, CNRS-Marseille, Aix-Marseille Université, Marseille, France;Laboratory for Experimental Study under Deep-sea Extreme Conditions, Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya, China;Laboratory of Deep-sea Microbial Cell Biology, Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya, China;University of Chinese Academy of Sciences, Beijing, China;

关键词: high hydrostatic pressure (HHP);    trimethylamine N-oxide (TMAO);    pressure tolerance;    Vibrio fluvialis;    Raman spectrometry;    CRISPRi;   

DOI  :  10.3389/fmicb.2017.02646

来源: DOAJ

【 摘 要 】

High hydrostatic pressure (HHP) exerts severe effects on cellular processes including impaired cell division, abolished motility and affected enzymatic activities. Transcriptomic and proteomic analyses showed that bacteria switch the expression of genes involved in multiple energy metabolism pathways to cope with HHP. We sought evidence of a changing bacterial metabolism by supplying appropriate substrates that might have beneficial effects on the bacterial lifestyle at elevated pressure. We isolated a piezosensitive marine bacterium Vibrio fluvialis strain QY27 from the South China Sea. When trimethylamine N-oxide (TMAO) was used as an electron acceptor for energy metabolism, QY27 exhibited a piezophilic-like phenotype with an optimal growth at 30 MPa. Raman spectrometry and biochemistry analyses revealed that both the efficiency of the TMAO metabolism and the activity of the TMAO reductase increased under high pressure conditions. Among the two genes coding for TMAO reductase catalytic subunits, the expression level and enzymatic activity of TorA was up-regulated by elevated pressure. Furthermore, a genetic interference assay with the CRISPR-dCas9 system demonstrated that TorA is essential for underpinning the improved pressure tolerance of QY27. We extended the study to Vibrio fluvialis type strain ATCC33809 and observed the same phenotype of TMAO-metabolism improved the pressure tolerance. These results provide compelling evidence for the determinant role of metabolism in the adaption of bacteria to the deep-sea ecosystems with HHP.

【 授权许可】

Unknown