The effect of cytokines produced by human adipose tissue on monocyte adhesion to the endothelium

Cell Adhesion & Migration

Sona Cejkova¹  Ivana Kralova Lesna¹  Rudolf Poledne¹  Hana Kubatova¹  Filip Thieme¹  Libor Janousek¹  Jiri Fronek¹ 

[1] Institute for Clinical and Experimental Medicine;

关键词: atherosclerosis;    cytokines;    inflammation;    endothelium;    adipose tissue;   

DOI  :  10.1080/19336918.2019.1644856

来源: DOAJ

【 摘 要 】

Visceral adipose tissue (VAT) may play a critical role in atherosclerotic cardiovascular disease. The goal of this study was to determine the effect of human VAT-released pro‑inflammatory cytokines on monocyte adhesion to the endothelium. The cytokine effects on monocyte adhesion to the endothelial cells (ECs) were tested using adipose tissue-conditioned media (ATCM) prepared by culturing human VAT. The cytokines concentrations in ATCM, the cytokines expression and adhesion molecules in stimulated ECs were measured. The concentrations of IL-1β,TNF-α,MCP-1,IL-10,and RANTES measured in ATCM correlated positively with monocyte adhesiveness to ECs. Additionally, ATCM increased the adhesion molecules (ICAM-1, VCAM-1) gene expression. Selective inhibitors highlighted the importance of IL-1β and TNF-α in the process by a significant decrease in monocyte adhesion compared to ATCM preconditioning without inhibitors. Human VAT significantly increased monocyte adhesion to ECs. It was significantly influenced by IL-1β, TNF-α, MCP-1, IL-10, and RANTES, with IL-1β and TNF‑α having the strongest impact.

【 授权许可】

Unknown