Dietary Copper Reduces the Hepatotoxicity of (−)-Epigallocatechin-3-Gallate in Mice

Molecules

Wenzheng Zhang¹  Najeeb Ahmed Kaleri²  Le Wang²  Xinghui Li²  Xuan Chen²  Jin Li²  Kang Sun² 

[1] Department of Regenerative and Cancer Cell Biology, Albany Medical College, Albany, NY 12208, USA;Tea Research Institute, Nanjing Agricultural University, Nanjing 210095, China;

关键词: tea;    (−)-epigallocatehin-3-gallate;    copper;    ceruloplasmin;    hepatotoxicity;   

DOI  :  10.3390/molecules23010038

来源: DOAJ

【 摘 要 】

We developed Cu-deficient, -sufficient and -super nutrition mice models by feeding them with diet containing 1.68, 11.72 or 51.69 mg of Cu/kg for 28 days, respectively. Then, the mice were treated to (−)-epigallocatechin-3-gallate (EGCG, 750 mg/kg BW) by oral in order to assess the acute toxicity of the drug. Following EGCG treatment, the survival rates were 12.5%, 50% and 100% in the Cu-deficient, -sufficient and Cu-super nutrition groups of mice, respectively. Cu level and ceruloplasmin activity in serum were significantly increased with the increase of dietary Cu. However, the Cu supplementation did not produce any obvious impact on serum superoxide dismutase activity. Furthermore, ceruloplasmin, in vitro, significantly promotes EGCG oxidation accompanied with increasing oxidation products and decreasing levels of reactive oxygen species. These results, therefore, suggest that Cu can relieve EGCG hepatotoxicity, possibly by up-regulating ceruloplasmin activity, which can be used to promote EGCG applications.

【 授权许可】

Unknown