In Vitro Synergy of Pongamia pinnata Extract in Combination with Antibiotics for Inhibiting and Killing Methicillin-Resistant Staphylococcus aureus

Antibiotics

Shun-Lai Li¹  Yi-Chung Lin¹  Ying-Chen Lu²  Chih-Cheng Lai³  Chi-Chung Chen⁴  Kuo-Chen Cheng⁵  Po-An Su⁵  Hung-Jen Tang⁵  Yin-Ching Chuang⁵ 

[1] Department of Biotechnology, Southern Taiwan University of Science and Technology, Tainan 71005, Taiwan;Department of Food Science, National Chiayi University, Chiayi 60004, Taiwan;Department of Internal Medicine, Kaohsiung Veterans General Hospital, Tainan Branch, Tainan 71051, Taiwan;Department of Medical Research, Chi Mei Medical Center, Tainan 71004, Taiwan;Department of Medicine, Chi Mei Medical Center, Tainan 71004, Taiwan;

关键词: antibiotics;    pharmaceuticals;    resistance;    staphylococcus;    antimicrobial;   

DOI  :  10.3390/antibiotics9030103

来源: DOAJ

【 摘 要 】

Aims: Currently, we face the serious problem of multiple drug-resistant pathogens. The development of new antimicrobial agents is very costly and time-consuming. Therefore, the use of medicinal plants as a source of alternative antibiotics or for enhancing antibiotic effectiveness is important. Methods: The antibacterial effects of aqueous extracts of the seed coat of Pongamia pinnata (Linn.) Pierre in combination with several antibiotics against methicillin-resistant Staphylococcus aureus (MRSA) were tested by broth dilution, checkerboard, and time-kill methods. Results: For the combinations of P. pinnata with ampicillin, meropenem, cefazolin, cefotaxime, cefpirome, and cefuroxime, 70% to 100% were synergistic, with a fractional inhibitory concentration (FIC) index of < 0.5. For the time-kill method with 0.5× minimum inhibitory concentration (MIC) of P. pinnata in combination with 8, 4, 2, and 1 µg mL⁻¹ of the various antibiotics, almost all of the combinations showed synergistic effects, even with the lowest concentrations of P. pinnata, except for aztreonam. No antagonistic effect was observed for these combinations. Conclusions: Based on these findings, aqueous seed coat extracts of P. pinnata have good potential for the design of new antimicrobial agents.

【 授权许可】

Unknown